# Utilization of Diamine Oxidase Enzyme from Mung Bean Sprouts (Vigna radiata L) for Histamine biosensors
> Karim A.

URL kanonis: https://discover.unhas.ac.id/publications/utilization-of-diamine-oxidase-enzyme-from-mung-bean-sprouts-vigna-radiata-l-for
Jurnal / Konferensi: Journal of Physics Conference Series
Tahun terbit: 2018
DOI: https://doi.org/10.1088/1742-6596/979/1/012014
ISSN: 17426588
Citations: 6

## Authors
- Karim A.

## Abstract
This research is aimed to utilize the diamine oxidase enzyme (DAO) which isolated from mung bean sprouts (Vigna radiata L) to develop histamine biosensors based on electode enzyme with the amperometric method (cyclic voltammetry).The DAO enzyme is trapped inside the membrane of chitin-cellulose acetate 2:1 and glutaraldehyde which super imposed on a Pt electrode. Histamine will be oxidized by DAO enzyme to produce aldehydes and H2O2 that acting as electron transfer mediators.The performance of biosensors will be measured at various concentrations of glutaraldehyde, temperature changes and different range of pH. Recently, it has been found that the optimal conditions obtained from the paramaters as follows; at 25% of glutaraldehyde, temperature of 37°C and pH of 7.4. Eventually, the results provided an expectation for applying histamine biosensors in determining the freshness and safety of fish specifically skombroidae families.

## Keywords
- Glutaraldehyde
- Diamine oxidase
- Biosensor
- Vigna
- Histamine
- Chemistry
- Enzyme
- Mung bean
- Immobilized enzyme
- Cellulose acetate
- Amperometry
- Enzyme assay
- Chromatography
- Nuclear chemistry
- Biochemistry
- Membrane
- Food science
- Electrode
- Biology
- Botany
- Electrochemistry
- Physical chemistry
- Endocrinology

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Sumber: Discover Unhas — RIMS Universitas Hasanuddin.
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