# Ultrasensitive and label-free biosensor for the detection of Plasmodium falciparum histidine-rich protein II in saliva
> Soraya G.V.

URL kanonis: https://discover.unhas.ac.id/publications/ultrasensitive-and-label-free-biosensor-for-the-detection-of-plasmodium-falcipar
Jurnal / Konferensi: Scientific Reports
Tahun terbit: 2019
DOI: https://doi.org/10.1038/s41598-019-53852-5
ISSN: 20452322
Kuartil SJR: Q1
Citations: 20

## Authors
- Soraya G.V.

## Abstract
Malaria elimination is a global public health priority. To fulfil the demands of elimination diagnostics, we have developed an interdigitated electrode sensor platform targeting the Plasmodium falciparum Histidine Rich Protein 2 (PfHRP2) protein in saliva samples. A protocol for frequency-specific PfHRP2 detection in phosphate buffered saline was developed, yielding a sensitivity of 2.5 pg/mL based on change in impedance magnitude of the sensor. This protocol was adapted and optimized for use in saliva with a sensitivity of 25 pg/mL based on change in resistance. Further validation demonstrated detection in saliva spiked with PfHRP2 from clinical isolates in 8 of 11 samples. With a turnaround time of ~2 hours, the label-free platform based on impedance sensors has the potential for miniaturization into a point-of-care diagnostic device for malaria elimination.

## Keywords
- Saliva
- Plasmodium falciparum
- Histidine
- Biosensor
- Protein detection
- Virology
- Computational biology
- Biochemistry
- Biology
- Chemistry
- Malaria
- Immunology
- Nanotechnology
- Amino acid
- Materials science

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Sumber: Discover Unhas — RIMS Universitas Hasanuddin.
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