The findings of escherichia coli in drinking water with reverse transcriptase polymerase chain reaction method at 16s rna gene

Abstract

Drinking water is the fundamental needs of urban communities. Emerging drinking water refilled station (DWRS) causing decreased in water quality so required the existence of quality monitoring efforts. RT-PCR technique could detect the presence of Escherichia coli in drinking water. The RT-PCR method is superior in accuracy, efficiency, and specificity. This research aims to analyze the presence of Escherichia coli as an indicator of the quality of refilled drinking water with the technique of RT-PCR target 16s RNA. The sample in this study was ten drinking water refilled station with the total sample 30 samples measured in the inlet, outlet and output. The results of RT-PCR in Mariso district, obtained RNA Band in the gene 16S RNA at position 723-bp in the sample a. 13. While in Panakukkang district captured RNA Band in the gene 16S RNA at position 723-bp on sample B. 11; B. 12; B. 13. Conclusions, Genomic RNA template by RT-PCR can be used to detect bacteria Escherichia coli in drinking water more quickly and accurately than conventional methods.

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