The effects of fermented raintree (Samanea saman) seed meal substitution in feed the digestive enzyme activity of pacific white shrimp Litopenaeus vannamei

Abstract

Abstract This research aimed to determine the effect of the substitution of soybean meal using raintree seed meal fermented with mixed microorganisms in feed, on the activity of protease, amylase, cellulase and lipase enzymes in pacific white shrimp (Litopenaeus vannamei) . The research used a completely randomized basic design, with five treatments and three replications at different levels of substitution of soybean meal with mixed microorganisms fermented raintree seed meal, namely 0, 25, 50, 75, and 100%. A total of 600 juvenile pacific white shrimp L. vannamei were reared for 60 days, with an initial weight of 0.08 g/fish, in an aquarium measuring 50x30x35 cm3, water volume 40 L, salinity 27 ppt, fed a dose of 10% of body weight with a frequency of four times a day at 06:00,12:00,18:00 and 21:00 WITA. The results showed that the enzyme activity of protease was 1.187, amylase 0.83, cellulase 111.57 and lipase 0.110 U/g/minute, optimal at 50% feed substitution.

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