# The Effect of Doxycycline on Matrix Metalloproteinase-9 Levels in the Colon of Wistar Rats with Obstructive Ileus
> Ulfandi D.

URL kanonis: https://discover.unhas.ac.id/publications/the-effect-of-doxycycline-on-matrix-metalloproteinase-9-levels-in-the-colon-of-w
Jurnal / Konferensi: Iium Medical Journal Malaysia
Tahun terbit: 2024
DOI: https://doi.org/10.31436/imjm.v23i01.2370
ISSN: 27352285
Kuartil SJR: Q4
Citations: 1

## Authors
- Ulfandi D.

## Abstract
INTRODUCTION: Obstructive ileus after colonic anastomosis resection can increase leakage up to 2.5-fold. One possibility could be the extracellular matrix degradation, where matrix metalloproteinase-9 (MMP-9) negatively influences anastomotic healing. This study aimed to determine the effect of doxycycline on MMP-9 levels in the colon of experimental rats with obstructive ileus. MATERIALS AND METHODS: In this study, 28 male Wistar rats were selected randomly in a post-test control-group design comprising: Group A - control group, Group B - group without obstructive ileus given doxycycline, Group C - group with obstructive ileus not given doxycycline, and Group D - group with obstructive ileus given doxycycline. Doxycycline was given at a dose of 30 mg/kg. Groups C and D underwent anastomotic resection 24 hours following obstructive ileus procedure. Groups A and B underwent anastomotic resection at the same time as Groups C and D. Colonic tissue was collected to examine MMP-9 levels using an enzyme-linked immunosorbent assay (ELISA). Data were compared using Shapiro–Wilk tests, ANOVA, and Mann–Whitney U tests, and p<0.05 was considered significant. RESULTS: The results showed that the average MMP-9 levels increased with doxycycline treatment, [Group C (4.294 ± 0.226)], with significantly higher MMP-9 levels than Group A (1.017 ± 1.569, p<0.001). Group B did not differ significantly from Group A (p=0.848). MMP-9 levels differed significantly between Groups B and D (p<0.001), and between Groups C and D (p<0.001). CONCLUSION: Oral administration of doxycycline can attenuate increasing MMP-9 levels in Wistar rats with obstructive ileus

## Keywords
- Medicine
- Doxycycline
- Ileus
- Anastomosis
- Gastroenterology
- Matrix metalloproteinase
- Internal medicine
- Group B
- Surgery
- Biochemistry
- Antibiotics
- Chemistry

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Sumber: Discover Unhas — RIMS Universitas Hasanuddin.
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