The effect of combined platelet-rich plasma and stromal vascular fraction compared with platelet-rich plasma, stromal vascular fraction, and vaseline alone on healing of deep dermal burn wound injuries in the Wistar rat

Abstract

Stromal Vascular Fraction cells (SVFs) are adipose tissue stem cells consisting of several types of cells, including Adipose Derivate Stem Cells (ADSCs). Platelet Rich Plasma (PRP) is a stem cell originating from blood that stimulates wound healing. This study aims to compare the acceleration of the healing process between a combination of PRP and SVFs, PRP, SVFs, and Vaseline using an animal model. This experimental study employed experimental method with post-treatment tests using a Wistar strain rat. The rats were divided into four groups: (1) PRP; (2) SVFs; (3) PRP and SVFs; and (4) Vaseline. Each group was given one treatment, and tissue samples were taken on days 0, 4, 7, 10, and 14. In the combined PRP and SVFs group, epithelialization, Polymorphonuclear (PMN) counts, fibroblast counts, and angiogenesis were found to be significantly increased. Compared with the other groups, the combined PRP and SVFs group was found in complete epithelialization on day 10 and slightly better at collagen synthesis compared to another groups. In the combined PRP and SVFs group, angiogenesis was found to be better than the other groups on day 4. PMN counts peaked on day 7 and began to decrease on day 10 in the PRP, SVFs, and the combined PRP and SVFs groups. Decrease of granulation mostly occurred on day 4 in the combined PRP and SVFs groups. There was a significant increase in epithelialization, tissue granulation, and collagen synthesis in the combined PRP and SVFs group compared to another group.

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