# The effect of administration of ethanol extract from Musa paradisiaca L. (MPL) fruit on the caspase-3 mrna gene expression in rat amyloid beta induced, an alzheimer’s disease model
> Kamelia E.

URL kanonis: https://discover.unhas.ac.id/publications/the-effect-of-administration-of-ethanol-extract-from-musa-paradisiaca-l-mpl-frui
Jurnal / Konferensi: Asian Journal of Pharmaceutical and Clinical Research
Tahun terbit: 2018
DOI: https://doi.org/10.22159/ajpcr.2018.v11i4.24103
ISSN: 09742441
Citations: 6

## Authors
- Kamelia E.

## Abstract
Objective: The objective of this study is to analyze caspase-3 mRNA gene expression in a Wistar rat model of Alzheimer’s disease (AD) treated with Musa paradisiaca L. (MPL) ethanol extract or banana extract (BE)Methods: MPL was evaluated for its effect on the caspase-3 mRNA gene expression of rat amyloid beta (Aβ) induced, an AD model. Each model included twenty Wistar rats were randomized into five groups: K0, without AD induction and no BE; K1, AD induction and no BE; K2, AD induction+BE 250 mg/kg body weight (BW); K3, AD induction+BE 500 mg/kg; and K4, AD induction+BE 1000 mg/kg. AD induction was performed by Aβ1-42 (0.2 ug) injection at the intracerebroventricular area. mRNA caspase-3 level measurements were performed by real time-polymerase chain reaction.Results: Paired t-test analysis showed no significant differences of caspase-3 mRNA level before Aβ induction among five groups (p>0.05). At 6 weeks post-Aβ induction, there was significantly increased caspase-3 mRNA in all groups except K0 (p<0.05). Notably, after 3 weeks of BE administration, caspase-3 mRNA expression was significantly decreased in all BE-treated groups; in the K1 placebo group, caspase-3 mRNA expression increased. The maximum decreased caspase-3 mRNA expression was in group K4 (-BE 1000 mg/kg BW), and the minimum was in group K2 (-BE 250 mg/kg BW).Conclusion: The results revealed that the ethanolic extract of MPL fruit could decrease caspase-3 mRNA gene expression in AD rat.

## Keywords
- Messenger RNA
- Gene expression
- Caspase 3
- Musa × paradisiaca
- Molecular biology
- Chemistry
- Pharmacology
- Apoptosis
- Internal medicine
- Endocrinology
- Biology
- Andrology
- Medicine
- Gene
- Biochemistry
- Programmed cell death
- Food science

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Sumber: Discover Unhas — RIMS Universitas Hasanuddin.
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