The Ability of Rot Fungi from Cocoa Plant in Producing Lignocellulosic Enzymes

Abstract

Abstract Rot fungi has the ability to degrade components of lignocellulosic which difficult to decompose. Rot fungi degrades components of cellulosic to get the nutrients and excrete the lignocellulosic enzyme. The study aims to examine the ability of rot fungi from decayed cocoa plant to grow on carbon compounds and produce the cellulosic enzyme. The capability of producing the enzyme performed by growing a piece of rot fungi isolates on Czapek Dox Agar media added Remazol dyes Briliant Blue 0.1%. The media was divided into 5 parts and each added one type of substrate lignocellulose: lignin, cellulose, pectin, amylose, and chitin as much as 0.1%. The formation of a clear zone around isolates indicative of enzyme activity. Scoring by measuring the magnitude of the clear zone formed in comparison to the area of petri dish. The results showed there were 9 kinds of rot fungi found in cocoa, namely Mycena sp, Lycoperdon sp, Auricularia sp, Schizophyllum sp, Coprinus sp, Tremella sp, Crepidotus sp, Tremetes sp, dan Pleurotus sp . Tremella sp has the highest ability to produce enzyme ligninase, kitinase, and pectinase, than all other isolates. Lycoperdon sp produces the highest cellulose enzyme, while the highest amylase founded on Pleurotus sp, Tremella sp, Schizophyllum sp, and .Crepidotus sp. .

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