# Symbiont bacteria cultures from the red algae Eucheuma spinosum, isolation of bioactive proteins and their anticancer potential test
> Ramadan R.

URL kanonis: https://discover.unhas.ac.id/publications/symbiont-bacteria-cultures-from-the-red-algae-eucheuma-spinosum-isolation-of-bio
Jurnal / Konferensi: Journal of Physics Conference Series
Tahun terbit: 2019
DOI: https://doi.org/10.1088/1742-6596/1341/3/032020
ISSN: 17426588
Citations: 3

## Authors
- Ramadan R.

## Abstract
Abstract Red algae Eucheuma spinosum is one of marine organisms which have the potential bioactive protein. This research aimed to determine the protein concentration of red algae Eucheuma spinosum and to discover its potential as an anticancer agent. Protein was isolated from bacterial symbiont of red algae Eucheuma spinosum by buffer A with pH value 8.3. Protein crude extract was fractionated by adding ammonium sulphate with a saturation level of 0-20%, 20-40%, 40-60%, and 60-80%. The result was dialyzed using cellophane membrane. Lowry method with Bovine Serum Albumin (BSA) was used as the standard to determine the protein level. An anticancer preliminary test was conducted using Brine Shrimp Lethality Test (BSLT) method. The result showed that the protein concentration from crude extract of red algae Eucheuma spinosum was 33.325 mg/mL. The highest concentration that was obtained at fraction 0-20% is 32.145 mg/mL. The result of toxicity test using Brine Shrimp Lethality Test (BSLT) method at protein fraction of 20-40% has a very low LC 50 value at <1000 µg/mL. Red algae protein fraction is potential to be developed as anticancer agent.

## Keywords
- Brine shrimp
- Stratum spinosum
- Biology
- Red algae
- Algae
- Bovine serum albumin
- Botany
- Biochemistry
- Fishery
- Genetics
- Stratum corneum

---
Sumber: Discover Unhas — RIMS Universitas Hasanuddin.
Saat mengutip, gunakan DOI bila tersedia atau URL kanonis di atas.