Selection of fungi that potentially produces IAA (Indole Acetic Acid) hormone origin of Takalar sugar factory waste

Abstract

Abstract This research aimed to obtain fungi isolates from the laboratory collection Mycology Department of Plant Pests and Diseases Hasanuddin University which has the potential to produce hormones Indole Acetic Acid (IAA). The isolate used is the result of isolation from Takalar sugar factory waste. Measurement The fungi isolate was inoculated in the media of Potato Dextrose Broth (PDB) enriched L-Tryptophan and then added the Salkowski Reagent. The results showed a change in pink color showed the fungi can produce the IAA hormone. High and low content of IAA produced by fungi isolates was shown with pink color. The pinker the suspense color, the higher the IAA content. IAA concentration produced by the isolates was measured using a spectrophotometer with a wavelength of 520 nm. The IAA value is calculated by comparing it with the absorbance value of the standard curve. The results showed that the isolate D4 code produced the highest IAA hormone concentration of 10.17 ppm.

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