# Sea grapes powder with the addition of tempe rich in collagen: An anti-aging functional food > Permatasari H.K. URL kanonis: https://discover.unhas.ac.id/publications/sea-grapes-powder-with-the-addition-of-tempe-rich-in-collagen-an-anti-aging-func Jurnal / Konferensi: F1000research Tahun terbit: 2022 DOI: https://doi.org/10.12688/f1000research.55307.3 ISSN: 20461402 Kuartil SJR: Q1 Citations: 6 ## Authors - Permatasari H.K. ## Abstract Background: This study aimed to determine the potential anti-aging effects of sea grapes and tempe (fermented soybeans) collagen particle size, by measuring the activities of anti-glycation, antioxidant, and tyrosinase inhibitors. Methods : Collagen was isolated from freeze-dried sea grapes and tempe powder and treated with different NaOH concentrations (0.10 M; 0.20 M; 0.30 M), and CH 3 COOH 1 M solution, separately. The collagen particle size was adjusted by stirring at 1000 rpm for 5 and 10 hours. 2,2-diphenyl-1-picrylhydrazyl (DPPH) was used to measure the antioxidant activity, and L-tyrosine and L-DOPA (l-3,4-dihydroxyphenylalanine) was used as a marker of tyrosine inhibition. Results: The collagen treated with 0.10 M NaOH produced the highest collagen yield (11.65%), and the largest particle size (2455 nm). Additionally, this collagen, when treated for 5 hours, exhibited 24.70% antioxidant activity, 62.60% anti-glycation, 8.97% L-tyrosine, and 26.77% L-Dopa inhibition activities. Meanwhile, the collagen treated for 10 hours had a 9.98% antioxidant activity, 41.48% anti-glycation, 7.89% L-tyrosine, and 2.67% L-Dopa inhibition activity. Conclusion: Sea grapes and tempe collagen powder treated with 0.10 M NaOH and stirred for 5 hours, possess the best potential anti-aging properties as a functional food. ## Keywords - Chemistry - Food science - Antioxidant - DPPH - Biochemistry --- Sumber: Discover Unhas — RIMS Universitas Hasanuddin. Saat mengutip, gunakan DOI bila tersedia atau URL kanonis di atas.