Rate Maturation, Fertilization and Hydrogen Peroxide Concentration of Bali Cow Oocytes with the Addition of Catalase and Hypotaurine

Abstract

This study was conducted to assess the effectiveness of catalase and hypotaurine on the rate of nuclear maturation, fertilization rate, and hydrogen peroxide concentration.This study was divided into three stages.The first stage is maturation media supplemented with catalase, the second stage is maturation media supplemented with hypotaurine, and the third stage is maturation and fertilization media supplemented with catalase, hypotaurine and a combination of both.This research employed compact cytoplasm and cumulus oocytes.After 24 hours in a 5% CO2 incubator at 38.5C, selected oocytes matured.After maturation, 1.5x10 6 spermatozoa/mL fertilization medium was used in a 5% CO2 incubator at 38.5C for in vitro fertilization.Oocytes were tested for hydrogen peroxide, nuclear maturation, and fertilization after 24 hours.The study indicated that 150IU/mL catalase led to the highest percentage of oocytes reaching metaphase II (75.43.3), with no significant difference (P>0.05) from other treatments.Adding 150IU/mL catalase lowered H2O2 concentration (16.6) (P<0.05)compared to the control (28).Hypotaurine increased oocyte MII to 6 mM (86.91.8) after addition, considerably different from the control (64.33.0)(P<0.05).The H2O2 concentration in the 6 mM treatment (22.9) was significantly lower (P<0.05)than the control (36.0).The maximum fertilization rate (78.62.7) was significantly (P<0.05)higher than the control (67.21.3) in the 150IU/mL and 6mM treatment.Hydrogen peroxide levels in the combination treatment (33.7) were significantly lower (P<0.05)than the control (47.1).In conclusion, catalase and hypotaurine supplementation promotes metaphase II and fertilized oocytes.

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