# RAPD primer screening for amplification on Katokkon pepper from Toraja, South Sulawesi, Indonesia
> Larekeng S.H.

URL kanonis: https://discover.unhas.ac.id/publications/rapd-primer-screening-for-amplification-on-katokkon-pepper-from-toraja-south-sul
Jurnal / Konferensi: Iop Conference Series Earth and Environmental Science
Tahun terbit: 2019
DOI: https://doi.org/10.1088/1755-1315/270/1/012023
ISSN: 17551307
Citations: 21

## Authors
- Larekeng S.H.

## Abstract
Abstract Random amplified polymorphic DNA (RAPD) is a well-known molecular marker that extensively applied to the genetic diversity analysis among species in the populations. Here, this marker was utilized in Katokkon pepper ( Capsicum chinense Jacq.), a highland pepper and having unique characteristics cultivated in specific regions (Tana Toraja and North Toraja), on preliminary primer screening using 30 RAPD primer pairs. A total of eight primers (OPA-02, OPAE-11, OPG-09, M-29, OPP-08, OPG-19, OPZ-05, and OPD-20) could produce polymorphic bands on twelve randomly selected DNA samples. OPA-02, OPP-08, OPG-19, OPZ-05, and OPD-20 generated polymorphic bands on all twelve evaluated samples, yet OPZ-05 was excluded in further analysis due to producing unclear bands. Clear and polymorphic bands were formed by OPAE-11 on eleven samples, whilst OPG-09 and M-29 only produced bands on ten samples. The primers are suitable to be used for amplifying DNA and determining the genetic diversity of Katokkon pepper in further analyses.

## Keywords
- RAPD
- Pepper
- Primer (cosmetics)
- Genetic diversity
- Biology
- Genetic marker
- Genetics
- Veterinary medicine
- Botany
- Traditional medicine
- Horticulture
- Medicine
- Gene
- Population
- Chemistry
- Organic chemistry
- Environmental health

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Sumber: Discover Unhas — RIMS Universitas Hasanuddin.
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