# Purification and characterization of α-glucosidase enzyme from rice groats
> Papriani N.P.

URL kanonis: https://discover.unhas.ac.id/publications/purification-and-characterization-of-glucosidase-enzyme-from-rice-groats
Jurnal / Konferensi: Journal of Physics Conference Series
Tahun terbit: 2019
DOI: https://doi.org/10.1088/1742-6596/1341/3/032017
ISSN: 17426588
Citations: 1

## Authors
- Papriani N.P.

## Abstract
Abstract Groat rice is the side product of rice milling process and potentially producing α-glucosidase. The enzyme has an important role in starch degradation process by breaking α-glycosidic bods to form glucose. This research aimed to understand the optimum activity of the purified α-glucosidase. The research stages are isolation, purification, characterization of substrate concentration, pH level, incubation time, temperature, and effect of metal ion, and dinitrosalicylic acid (DNS) method to determine the activity of α-glucosidase. The result showed the specific activity of α-glucosidase crude extract at 0.003 IU/mg. Fractionation of the highest specific activity on fraction 4 revealed at 0.042 IU/mg and constantly increasing on the dialysis stage at 1.121 IU/mg. α-glucosidase reached optimum activity at substrate concentration 2%, pH of 7, incubation time of 40 minutes, at 37°C, with metal ion Mn 2+ , Co 2+ , Na + , Mg 2+ , Ca 2+ and K + as activators and Zn 2+ as inhibitor. α-glucosidase could be used to find analog compounds as the cure of type 2 diabetes mellitus.

## Keywords
- Chemistry
- Incubation
- Substrate (aquarium)
- Enzyme
- Starch
- Chromatography
- Fractionation
- Hydrolysis
- Enzyme assay
- Metal ions in aqueous solution
- Metal
- Nuclear chemistry
- Food science
- Biochemistry
- Organic chemistry
- Biology
- Ecology

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Sumber: Discover Unhas — RIMS Universitas Hasanuddin.
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