The effect of BAP on the tissue culture of Melaleuca cajuputi

Abstract

Abstract Melaleuca cajuputi is a non-wood-producing tree that benefits both as a greening plant and as an M. cajuput oil-producing plant. Efforts to support the increase in the need for M. cajuputi begin by knowing the propagation techniques used. Propagation of M. cajuputi generatively and vegetatively by cuttings takes a relatively long time, so modern vegetative propagation is carried out, namely tissue culture. Multiplication is one of the stages of tissue culture with the primary goal of bud propagation. Plant Growth Regulators (PGRs) greatly influence the success of multiplication in the media. Benzyl Amino Purine (BAP) is a Growth Regulator that significantly influences bud formation. This study aims to obtain the right concentration of PGRs in the form of BAP to support the multiplication process of M. cajuputi . The research method starts from the sterilization stages of the instrument, making media, initiation of M. cajuputi seeds, multiplication, and observation. The data obtained were analyzed by Variance (ANOVA) and correlation tests using the SPSS program and Principal Component Analysis (PCA) using Minitab Statistical Software. The findings showed that Murashige and Skoog (MS) media with PGRs added at BAP 1 ppm levels was the best medium for M. cajuputi to grow or multiply in a lab setting in order to form buds.

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