Genetic variability of mud crab Scylla tranquebarica from Malili, South Sulawesi based on mitochondrial 16S rRNA sequence: A preliminary assessment

Abstract

Abstract Analysis of genetic variability is a very important aspect of providing the genetic database for the domestication purposes of mud crab Scylla tranquebarica. Genetic characterization is needed to be assessed to support the mud crab breeding program. The study aimed to analyze the genetic variation of the mud crab S. tranquebarica broodstock collected from Malili, East Luwu, South Sulawesi, based on the nucleotide sequence of mitochondrial 16S rRNA. The mud crab samples (n=10) were collected from fishermen. The genomic DNA and mtDNA 16S rRNA were isolated by the commercial kits, and the nucleotide sequence was done in the First Base. The sequences of mtDNA were analyzed using Mega-X, BLAST-N, and DnaSP to obtain a consensus sequence, haplotype diversity, nucleotide diversity, haplotype number, and genetic distance. The results of the study revealed the genetic variability of mud crabs from Malili in terms of relatively high haplotype diversity of 0.533, low nucleotide diversity of 0.00096, and two haplotypes. Genetic distance among individuals for intra-population was 0.0010±0.0009. The two haplotypes were highly correlated with the number of clusters observed. Two clusters were detected in which the 1st cluster consisted of six individuals, and the 2nd cluster consisted of four individuals.

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