# Mechanistic Elucidation of green seaweed compounds in orthodontic relapse management via RANKL/TNF-α-mediated ROS/Keap1/Nrf2 signaling: In silico and Ex Vivo studies
> Alhasyimi A.A.

URL kanonis: https://discover.unhas.ac.id/publications/pub_scopus_85203184456
Jurnal / Konferensi: Journal of Agriculture and Food Research
Tahun terbit: 2024
DOI: https://doi.org/10.1016/j.jafr.2024.101396
ISSN: 26661543
Kuartil SJR: Q1
Citations: 2

## Authors
- Alhasyimi A.A.

## Abstract
Orthodontic relapse, the return to a pre-treatment position after orthodontic correction, is driven by the RANKL/TNF-α-mediated ROS/Keap1/Nrf2 signaling axis. This mechanism triggers aseptic inflammation and oxidative stress, influencing bone resorption and formation. Antioxidants can mitigate oxidative stress, potentially improving post-orthodontic outcomes. This study explores the efficacy of antioxidant compounds derived from green seaweed/algae in managing orthodontic relapse. Green seaweed/algae extracts were prepared via sonication, and bioactive compounds were identified using ultra-performance liquid chromatography-electrospray ionization-tandem mass spectrometry (UPLC-ESI-MS/MS) analysis. Compounds underwent bioactivity prediction, toxicity assessment, and drug-likeness evaluation, revealing significant therapeutic potential. Network pharmacology and molecular docking identified key proteins associated with orthodontic relapse, including IL-1β, STAT3, ESR1, MAPK1, JAK2, and HMOX1. Molecular docking simulations indicated favorable binding energies for green seaweed compounds, particularly the alkaloids adenosine (ΔG −6.9 to −7.3 kcal/mol) and lycopodine (ΔG −6.3 to −8.5 kcal/mol), against targeted proteins, matching or outperforming standard drugs such as s-ibuprofen (ΔG −6.7 kcal/mol). In vitro assays confirmed the antioxidant activity of these compounds, with EC 50 dose of 52.2–54.2 μg/mL for ABTS radical scavenging capacities. Protein expression analysis in tibial-femoral bone marrow cells further demonstrated the potential of green seaweed/algae compounds to suppress osteoclastogenesis by modulating the RANKL/TNF-α-mediated ROS/Keap1/Nrf2 pathway. This research highlights the promise of green seaweed-derived antioxidants in reducing oxidative stress and managing orthodontic relapse, providing a foundation for future therapeutic developments. • Green seaweed potential in reducing oxidative stress and preventing orthodontic relapse. • Green seaweed suppresses osteoclastogenesis by modulating the RANKL/TNF-α-mediated ROS/Keap1/Nrf2 pathway. • Pharmacologists and clinicians must collaborate to bring promising food functional findings from the bench to the bedside.

## Keywords
- In silico
- Ex vivo
- RANKL
- Tumor necrosis factor alpha
- In vivo
- KEAP1
- Chemistry
- Cell biology
- Cancer research
- Medicine
- Biology
- Immunology
- Biochemistry
- Receptor
- Gene
- Genetics
- Transcription factor
- Activator (genetics)

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Sumber: Discover Unhas — RIMS Universitas Hasanuddin.
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