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Effects of platelet-rich plasma and stromal vascular factor combination on fibroblast count in anal trauma in Wistar rats
Richardo S.
Gazzetta Medica Italiana Archivio Per Le Scienze Mediche
Q4Abstract
BACKGROUND: Fibroblasts are most commonly found in the dermis and have a role in the proliferative phase of wound healing. Injuries stimulate fibroblasts to proliferate and migrate to the wound area. Many treatments have been developed for managing anal trauma injuries, including using a combination of platelet-rich plasma (PRP) and stromal vascular factor (SVF). Studies have suggested that wound healing may be faster with a combined PRP and SVF treatment. We assessed the effect on an anal trauma model by evaluating fibroblast count.METHODS: Twenty-eight Wistar rats were divided into three groups: group A (Wistar with anal trauma injected with a combination of SVF and PRP), group B (Wistar with anal trauma without therapy), and group C (control group). These groups were further divided based on the day of sacrifice (days 1, 7, and 14). Fibroblast counts were analyzed using histopathological examination. Repeated ANOVA tests were used, and P-values of <0.05 were considered statistically significant.RESULTS: The highest mean fibroblast counts were in group A, then groups B and C (123.25±9.35 units; 105.50±25.41 units; 91.75±16.11 units). The highest fibroblast count was on day 7, during the proliferative phase of wound healing. From the repeated ANOVA Test, the p-value of 0.034 indicates a significant general difference in fibroblast count by group.CONCLUSIONS: The combination of PRP and SVF can improve the wound healing process based on the fibroblast count in an anal trauma model using Wistar rats. A significant difference existed in the average fibroblast count by day between groups.