# Antibiofilm Activity of Tapak Kuda Ipomoea pescaprae against Pseudomonas aeruginosa ATCC 27853 and Methicillin-Resistant Staphylococcus aureus (MRSA) ATCC 43300: In-Vitro and In-Silico Evaluation
> Baktiar N.H.

URL kanonis: https://discover.unhas.ac.id/publications/pub_scopus_85174342195
Jurnal / Konferensi: Journal of Pure and Applied Microbiology
Tahun terbit: 2023
DOI: https://doi.org/10.22207/JPAM.17.3.44
ISSN: 09737510
Kuartil SJR: Q3
Citations: 4

## Authors
- Baktiar N.H.

## Abstract
Ipomoea pes-caprae is one of the herbal plants that can treat various health problems such as skin infections, burns, boils, and various diseases caused by microbial infections. This study aims to identify ethanol extract compounds of I. pes-caprae leaf and evaluate their antibiofilm activity through in-vitro and in-silico assays. This study used two test bacteria, Pseudomonas aeruginosa and Methicilin Resistant Staphylococcus aureus. Antibacterial activity is carried out using the agar diffusion method and antibiofilm using a microplate reader. The biological activity was also evaluated through a computational approach using molecular docking. The results of preliminary test demonstrated the antibacterial activity. At a concentration of 100 mg/mL, I. pes-caprae extract produced a substantial inhibitory zone of 13.9 mm for P. aeruginosa and a moderate 8.5 mm zone for MRSA. The extract also showed high antibiofilm activity. It achieved impressive biofilm inhibition rates of 82.58% and 78.29%, respectively. Molecular docking shows the interaction between extract compounds and macromolecules that play a role in biofilm formation, namely SrtA and associated protein biofilms. 1,3,4,5-Tetrahydroxy-Cyclohexanecarboxylic Acid has the lowest binding energy of -7.5 Kcal/Mol and -5.9 Kcal/Mol at each target receptor. This study demonstrated the antibiofilm potential of I. pes-caprae extract, which was clarified through molecular docking studies.

## Keywords
- Staphylococcus aureus
- Pseudomonas aeruginosa
- Microbiology
- Biofilm
- Antibacterial activity
- Minimum inhibitory concentration
- Agar diffusion test
- Methicillin-resistant Staphylococcus aureus
- Chemistry
- Agar
- In vitro
- Biology
- Antimicrobial
- Bacteria
- Biochemistry
- Genetics

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Sumber: Discover Unhas — RIMS Universitas Hasanuddin.
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