# Genome-wide functional screening of drug-resistance genes in Plasmodium falciparum
> Iwanaga S.

URL kanonis: https://discover.unhas.ac.id/publications/pub_scopus_85140149011
Jurnal / Konferensi: Nature Communications
Tahun terbit: 2022
DOI: https://doi.org/10.1038/s41467-022-33804-w
ISSN: 20411723
Kuartil SJR: Q1
Citations: 6

## Authors
- Iwanaga S.

## Abstract
The global spread of drug resistance is a major obstacle to the treatment of Plasmodium falciparum malaria. The identification of drug-resistance genes is an essential step toward solving the problem of drug resistance. Here, we report functional screening as a new approach with which to identify drug-resistance genes in P. falciparum. Specifically, a high-coverage genomic library of a drug-resistant strain is directly generated in a drug-sensitive strain, and the resistance gene is then identified from this library using drug screening. In a pilot experiment using the strain Dd2, the known chloroquine-resistant gene pfcrt is identified using the developed approach, which proves our experimental concept. Furthermore, we identify multidrug-resistant transporter 7 (pfmdr7) as a novel candidate for a mefloquine-resistance gene from a field-isolated parasite; we suggest that its upregulation possibly confers the mefloquine resistance. These results show the usefulness of functional screening as means by which to identify drug-resistance genes.

## Keywords
- Plasmodium falciparum
- Drug resistance
- Genome
- Gene
- Biology
- Genetics
- Computational biology
- Drug
- Malaria
- Virology
- Pharmacology
- Immunology

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Sumber: Discover Unhas — RIMS Universitas Hasanuddin.
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