Physiological Response and Detection of Inh2 Gene in Dieng Red Potato (Solanum tuberosum L.) Affected by Frost

Abstract

In this study, the response of Dieng red potato plants to frost exposure was evaluated physiologically by testing electrolyte leakage and chlorophyll content, and molecularly by detection of Inh2 gene.The physiological response shows an increased electrolyte leakage rate and a significant decrease in chlorophyll content in plants exposed to frost compared to those grown in normal temperatures.Sequencing of Inh2 gene identifies four homologous Solanum tuberosum alleles.The difference in the order of Inh2 lies in the gap of 12 bp.Phylogenetic analysis shows that inh2 gene is in a cluster with INH2α*C allele and has evolved slowly from a common ancestor with S. lycopersicum.This is the first study on the physiological mechanisms of frost tolerance and the sequencing of Inh2 gene in Dieng red potatoes.Hopefully, this study will provide useful information for the breeding of low-sugar Dieng red potatoes.

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