# Mitochondrial Cytochrome Oxidase Subunit 1: A Promising Molecular Marker for Species Identification in Foraminifera
> Girard E.B.

URL kanonis: https://discover.unhas.ac.id/publications/pub_scopus_85124710189
Jurnal / Konferensi: Frontiers in Marine Science
Tahun terbit: 2022
DOI: https://doi.org/10.3389/fmars.2022.809659
ISSN: 22967745
Kuartil SJR: Q1
Citations: 15

## Authors
- Girard E.B.

## Abstract
Traditional morphological methods for species identification are highly time consuming, especially for small organisms, such as Foraminifera, a group of shell-building microbial eukaryotes. To analyze large amounts of samples more efficiently, species identification methods have extended to molecular tools in the last few decades. Although a wide range of phyla have good markers available, for Foraminifera only one hypervariable marker from the ribosomal region (18S) is widely used. Recently a new mitochondrial marker cytochrome oxidase subunit 1 (COI) has been sequenced. Here we investigate whether this marker has a higher potential for species identification compared to the ribosomal marker. We explore the genetic variability of both the 18S and COI markers in 22 benthic foraminiferal morphospecies (orders Miliolida and Rotaliida). Using single-cell DNA, the genetic variability within specimens (intra) and between specimens (inter) of each species was assessed using next-generation sequencing. Amplification success rate was twice as high for COI (151/200 specimens) than for 18S (73/200 specimens). The COI marker showed greatly decreased intra- and inter-specimen variability compared to 18S in six out of seven selected species. The 18S phylogenetic reconstruction fails to adequately cluster multiple species together in contrast to COI. Additionally, the COI marker helped recognize misclassified specimens difficult to morphologically identify to the species level. Integrative taxonomy, combining morphological and molecular characteristics, provides a robust picture of the foraminiferal species diversity. Finally, we suggest the use of a set of sequences (two or more) to describe species showing intra-genomic variability additionally to using multiple markers. Our findings highlight the potential of the newly discovered mitochondrial marker for molecular species identification and metabarcoding purposes.

## Keywords
- Biology
- Molecular marker
- Foraminifera
- Cytochrome c oxidase subunit I
- Phylogenetic tree
- DNA barcoding
- Evolutionary biology
- Genetic marker
- Ribosomal DNA
- Hypervariable region
- Ribosomal RNA
- Genetic diversity
- 18S ribosomal RNA
- Genetics
- Ecology
- Gene
- Benthic zone
- Population
- Sociology
- Demography

---
Sumber: Discover Unhas — RIMS Universitas Hasanuddin.
Saat mengutip, gunakan DOI bila tersedia atau URL kanonis di atas.