Amplification of 16S rRNA primer on rhizosphere bacteria from reclamation area and natural forest of PT. Vale

Abstract

Abstract Open-pit mining activities cause land degradation; therefore, post-mining land recovery or reclamation is essential. An indicator to rate the success of mine reclamation activities is the diversity of soil microbial populations. Molecular bacteria identification requires prior information about the amplification of universal primers for molecular analysis. This study analyzes the amplification of 16S rRNA primers on rhizosphere bacteria isolates from reclamation and forest areas. The research activities encompassed sample preparation, isolation of bacteria isolates, isolation of DNA isolate, quantitative test, qualitative test, and amplification. The findings showed that pure isolates of rhizosphere bacteria from reclamation and natural forest areas that could be used were five and ten isolates, respectively. One isolate (20%) from the reclamation area and four isolates (40%) from natural forest generated DNA band, which were amplified using 16S rRNA.

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