# The Terpenoid Activity of Ethanol extracted from Purple Yam Sap to Inhibit the Growth of R. oligosporus and S. cerevisiae
> Nurfitriani A.

URL kanonis: https://discover.unhas.ac.id/publications/pub_scopus_85116461000
Jurnal / Konferensi: International Journal on Advanced Science Engineering and Information Technology
Tahun terbit: 2021
DOI: https://doi.org/10.18517/ijaseit.11.4.12336
ISSN: 20885334
Kuartil SJR: Q4
Citations: 0

## Authors
- Nurfitriani A.

## Abstract
Plants that contain secondary metabolite components can be used as an anti-microbial. Terpenoids are part of secondary metabolic, which is naturally in the plants. This study aims to investigate the terpenoid activity of purple yam sap as an anti-microbe to prevent the growth of <em>R. Oligosporus’</em>mold and <em>S.Cerevicea’s yeast. </em>The terpenoid property within the purple <em>yam sap</em> was identified using the thin-layer chromatography (TLC) eluent toluene of <em>Etil Asetat </em>(3: 7). The anti-microbial activity was tested using the agar diffusion method, and the cell damage analysis was carried out using SEM. This study showed that the anti-microbial activities of the terpenoid to inhibit the growth of <em>R. Oligosporus </em>mold were as follow: the 96% ethanol extract had the inhibition zone of 8.5 mm, the 80% ethanol extract had the inhibition zone of 9.5 mm, and 65% ethanol extract had the inhibition zone of 10.03 mm, whereas the 50% ethanol extract had the highest inhibition zone by 10.93 mm. Meanwhile, 96% ethanol extract had the most robust ability to inhibit the growth of <em>S.crevicea</em> yeast by 11.07 mm, and 80% of the ethanol extract had the weakest ability to inhibit the growth of this yeast only 9.23 mm diameter of inhibition zone. The terpenoids substance with the minimum concentrate (0.01%). Extract causes the cell of the <em>R.oligosporus</em> fungus and cells the <em>S. Cereviceae’s</em> yeast to leak; thus, the cell ruptured and died. On the one hand, the <em>S.cereviceae</em> cell changes the shapes and experiences cell damage.

## Keywords
- Terpenoid
- Rhizopus oligosporus
- Ethanol
- Chemistry
- Yeast
- Fermentation
- Agar diffusion test
- Food science
- Chromatography
- Biochemistry
- Botany
- Biology
- Escherichia coli
- Gene

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Sumber: Discover Unhas — RIMS Universitas Hasanuddin.
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