# Evaluation of antioxidant activity of botto-botto leaf fraction (Chromolaena odorata l.) using dpph and abts methods
> Tahir K.A.

URL kanonis: https://discover.unhas.ac.id/publications/pub_scopus_85105429244
Jurnal / Konferensi: Open Access Macedonian Journal of Medical Sciences
Tahun terbit: 2021
DOI: https://doi.org/10.3889/oamjms.2021.5982
ISSN: 18579655
Citations: 5

## Authors
- Tahir K.A.

## Abstract
BACKGROUND: Antioxidants are substances that can inhibit the oxidation process, so that they have a protective effect on cells from the dangers of free radicals. One of the plants that has activity as natural antioxidants is Botto-Botto leaves (Chromolaena odorata L.). AIM: This study aims to determine the antioxidant activity of the Botto-Botto leaf fraction using the DPPH and ABTS methods. METHODS: The leaves of Botto-Botto (Chromolaena odorata L.) obtained from Takalar District, South Sulawesi are sorted, dried and pollinated. The powder was immersed in 70% ethanol solvent and sonicated. Furthermore, the extraction was carried out by maceration method with 70% ethanol solvent and fractionated using water, n-hexane, and n-butanol as solvents and dried. The fractionation results were continued for antioxidant testing using the DPPH and ABTS methods. RESULTS: The n-butanol fraction of Botto-Botto leaves had the highest antioxidant activity, namely the strong active category. In the DPPH and ABTS methods, the n-butanol fraction has IC50 values of 33.535 μg / mL and 60.885 μg / mL, respectively. CONCLUSION: The DPPH and ABTS methods, the n-butanol fraction of botto-botto leaves have strong antioxidant activity compared to other fractions, namely the water fraction and the n-hexane fraction.

## Keywords
- ABTS
- DPPH
- Chromolaena odorata
- Antioxidant
- Fraction (chemistry)
- Butanol
- Maceration (sewage)
- Fractionation
- Chromatography
- Traditional medicine
- Food science
- Ethanol
- Chemistry
- Botany
- Organic chemistry
- Medicine
- Biology
- Materials science
- Weed
- Composite material

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Sumber: Discover Unhas — RIMS Universitas Hasanuddin.
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