# Efficacy of Photodynamic Anti-Microbial Chemotherapy for Acanthamoeba Keratitis In Vivo
> Dwia Pertiwi Y.

URL kanonis: https://discover.unhas.ac.id/publications/pub_scopus_85097314025
Jurnal / Konferensi: Lasers in Surgery and Medicine
Tahun terbit: 2021
DOI: https://doi.org/10.1002/lsm.23355
ISSN: 01968092
Kuartil SJR: Q2
Citations: 15

## Authors
- Dwia Pertiwi Y.

## Abstract
BACKGROUND AND OBJECTIVES: Acanthamoeba keratitis is a sight-threatening infectious disease that is difficult to treat. The aim of this study was to evaluate TONS504 (cationic chlorin derivative photosensitizer)-mediated photodynamic antimicrobial chemotherapy (PACT) in vivo as a potential treatment for Acanthamoeba keratitis. STUDY DESIGN/MATERIALS AND METHODS: /ml Acanthamoeba castellanii, which were placed over debrided corneas with temporary tarsorrhaphy. Thirty-eight male Japanese white rabbits were randomly divided into three groups (normal eye, no treatment, and treatment groups). TONS504 was administered as eye drops at 1 mg/ml, followed by light-emitting diode irradiation after the establishment of keratitis at 7 days after infectious contact lens exposure. All animals were evaluated under a slit-lamp microscope every 3 days for 6 days after the treatment. Clinical scores based on corneal epithelial defects detected by fluorescein staining, stromal opacity edema, and vascular infiltration into the cornea were determined. After 6 days, the eyes were enucleated for histopathological analysis. RESULTS: Clinical signs of infection in the treatment group were markedly reduced for up to 6 days after treatment. Histopathology showed a regular arrangement of stromal fibers and a small number of inflammatory cells in 58% of the corneas. However, 42% of corneas in the treatment group showed infiltrating neutrophils and irregular alignment of stromal collagen fibers. CONCLUSIONS: Our TONS504-PACT achieved complete recovery from keratitis in 58% of the rabbit models. Further studies are required to determine the conditions for the maximal effectiveness of our TONS504-PACT for Acanthamoeba keratitis. Lasers Surg. Med. © 2020 Wiley Periodicals LLC.

## Keywords
- Acanthamoeba keratitis
- Acanthamoeba
- Keratitis
- Cornea
- Medicine
- Contact lens
- In vivo
- Photodynamic therapy
- Histopathology
- Ophthalmology
- Pathology
- Surgery
- Microbiology
- Biology
- Chemistry
- Organic chemistry
- Biotechnology

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Sumber: Discover Unhas — RIMS Universitas Hasanuddin.
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