Bacterial diversity in superintensive vanname shrimp aquaculture wastewater treatment plants in Barru and Takalar Regencies

Abstract

Abstract Super intensive wastewater treatment plant (WWTP) is needed to manage wastewater from aquaculture activities before being discharged into the waters. This study aims to determine the diversity of bacterial species in the WWTP super intensive vanname shrimp culture plots located in Barru and Takalar Regencies. The research method was designed by using a survey method in the WWTP super-intensive shrimp cultivation plot in Lajange Village, Mallusetasi District, Barru Regency, and Punanga Village, Takalar Regency. Sampling of DNA from water was carried out every two weeks for one culture cycle by filtering water from the WWTP outlet. The samples obtained were then DNA-extracted using cTAB dTAB DNA extraction kit (Iq2000). Quality of the DNA obtained was measured and analyzed using a genquan machine. Next Generation Sequencing (NGS) analysis was carried out by sending the DNA samples to a commercial company (Genetics Science laboratory) for the analysis. The results showed that the highest relative diversity of samples from Barru was 60% unidentified, followed by vibrio, unidentified Chloroplast, Arcobacter, Pseudoalteromonas, Marinobacterium, Marivita, Thalassatolea, Phaeodactylibacter, and Thalassalituus. Likewise, the relative diversity of bacteria from Takalary originating from Takalar, the highest was also not identified, followed by Pseudoalteromonas, unidentified_Chloroplast, Vibrio, Marivita, Thalassotalea, Phaedactylibacter, Thalassolituus, Arcobacter, and Tenacibaculum. It appears that the types of bacteria from the two sampling locations are still quite diverse and found probiotic bacteria, Pseudoalteromonas, that are commonly used in the cultivation of vanname shrimp in both WWTP plots.

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