# Effects of lime (Citrus aurantifolia) peel to the expression of mRNA toll-like receptors 4 in balb/c mice-infected Salmonella typhi
> Kasim V.N.

URL kanonis: https://discover.unhas.ac.id/publications/pub_scopus_85094594399
Jurnal / Konferensi: Journal of Advanced Pharmaceutical Technology and Research
Tahun terbit: 2020
DOI: https://doi.org/10.4103/japtr.JAPTR_48_20
ISSN: 22314040
Kuartil SJR: Q3
Citations: 13

## Authors
- Kasim V.N.

## Abstract
Lime peel contains metabolic compounds that have lethal effects of bacterial cells, but its effect as an antibacterial modulate innate immunity pathways, especially toll-like receptor 4 (TLR-4) signaling pathway, is unclear. This study examined the effects of lime peel extract (LPE) on the activity of TLR 4 in Balb/c mice induced by Salmonella typhi. Mice were induced intraperitoneally and then 3 days after induction, LPE was given orally on two doses (510 and 750 mg/kg BW). The number of bacterial colonization was counted using peritoneal fluid samples by the method of plate count agar. Intervention LPE for 5 days can degrade TLR-4 and the number of colonies of S. typhi. On day 3 after was induced S. typhi, TLR-4 gene expression of Balb/c mice is increased. Postintervention LPE for 5 days, the expression of TLR-4 gene decreased, significantly different at a dose of 750 mg/kg BW (P = 0.04). There was a positive correlation between the expression of TLR-4 gene by the number of bacterial colonization, decreasing gene expression of TLR-4, the number of bacterial colonization is also getting smaller (P= 0.013, r = 0.408). LPE can modulate the TLR-4 signaling pathway in host immunity so that the gene TLR-4 is expressed fewer in numbers. This mechanism causes the bacterial colony number to decrease, not even growth.

## Keywords
- Microbiology
- Biology
- Salmonella typhi
- Gene expression
- Innate immune system
- Immunity
- Salmonella
- Receptor
- BALB/c
- Gene
- Immune system
- Immunology
- Bacteria
- Escherichia coli
- Biochemistry
- Genetics

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Sumber: Discover Unhas — RIMS Universitas Hasanuddin.
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