Isolation, characterization and anticancer potential test of crude extract of L-asparaginase enzyme from siam weed leaf (Chromolaena odorata Linn): A novel source

Abstract

Abstract L-asparaginase enzyme is potentially isolated from Siam weed (C. odorata L.) and also used as anticancer treatment. The point of this study is to isolate crude extract of L-asparaginase as well as its characteristics for anticancer. The activity of the enzyme was observed by Nessler Method and its potential for anticancer was tested by Brine Shrimp Lethality Test (BSLT) method. The results showed the specific activity by 4.8234 UI/mg. The enzyme worked maximally at pH of 8, 37 °C for 30 minutes with Na+ and K+ being activator and Ca2+, Zn+2, Mg+2, Cu+2, Co+2, Mn+2 as inhibitor. Base on BSLT revealed an LC50 value of 5.8063 μg/mL and proved that the enzyme has biotoxicity in high level. The results of this study denoted that C. odorata L. leaves able to be isolated and characterized by L-asparaginase enzyme and these reveals the potential to be developed as an anticancer in the future.

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