# Down-selecting circumsporozoite protein-based malaria vaccine: A comparison of malaria sporozoite challenge model
> Amelia F.

URL kanonis: https://discover.unhas.ac.id/publications/pub_scopus_85064349331
Jurnal / Konferensi: Parasite Immunology
Tahun terbit: 2019
DOI: https://doi.org/10.1111/pim.12624
ISSN: 01419838
Kuartil SJR: Q2
Citations: 11

## Authors
- Amelia F.

## Abstract
Plasmodium falciparum circumsporozoite protein (PfCSP) is the main target antigen in development of pre-erythrocytic malaria vaccines. To evaluate PfCSP vaccines in animal models, challenge by intravenous sporozoite injection is preferentially used. However, in clinical trials, vaccinated human volunteers are exposed to the bites of malaria-infected mosquitoes. In this study, we down-selected Escherichia coli-produced full-length PfCSP (PfCSP-F) and its three truncated PfCSPs based on their abilities to elicit immune response and protection in mice against two challenge models. We showed that immunization with three doses of PfCSP-F elicited high anti-PfCSP antibody titres and 100% protection against the bites of infected mosquitoes. Meanwhile, three-dose truncated PfCSP induced 60%-70% protection after immunization with each truncated PfCSP. Heterologous prime-boost immunization regimen with adenovirus-PfCSP-F and R32LR greatly induced complete protection against intravenous sporozoite injection. Our results suggest that Abs to both anti-repeat and anti-nonrepeat regions induced by PfCSP-F are required to confer complete protection against challenge by the bites of infected mosquitoes, whereas anti-repeat Abs play an important role in protection against intravenous sporozoite injection. Our findings provide a potential clinical application that PfCSP-F vaccine induces potent Abs capable of neutralizing sporozoites in the dermis inoculated by infected mosquitoes and subsequently sporozoites in the blood circulation.

## Keywords
- Circumsporozoite protein
- Malaria
- Biology
- Malaria vaccine
- Virology
- Immunology
- Plasmodium (life cycle)
- Plasmodium falciparum
- Parasite hosting
- Computer science
- World Wide Web

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Sumber: Discover Unhas — RIMS Universitas Hasanuddin.
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