# The use of response surface method in optimization of levan production by heterologous expressed levansucrase from halophilic bacteria bacillus licheniformis BK2
> Permatasari N.U.

URL kanonis: https://discover.unhas.ac.id/publications/pub_scopus_85059533183
Jurnal / Konferensi: Iop Conference Series Earth and Environmental Science
Tahun terbit: 2018
DOI: https://doi.org/10.1088/1755-1315/209/1/012015
ISSN: 17551307
Citations: 7

## Authors
- Permatasari N.U.

## Abstract
Response surface method (RSM) is a recent common method used to identify culture condition for optimal production of particular metabolite. In the present study, RSM is used to optimize a catalytic reaction in levan production by heterologous expression of lsbl-bk2 gene isolated from halophilic bacteria Bacillus licheniformis . Levan is a polyfructose polymer produced from sucrose by the action of extracellular levansucrase secreted by the microorganism. Three factors for levan production, namely sucrose concentration, pH, and temperature of reaction were optimized by full factorial and central composite designs in RSM. The result indicated that the optimum in vitro condition for levan production was achieved when the levansucrase catalytic reaction was performed at 32°C, pH 8, in 12% (w/v) sucrose solution. Levan produced by this procedure was verified by FTIR and NMR spectroscopies.

## Keywords
- Levansucrase
- Bacillus licheniformis
- Response surface methodology
- Sucrose
- Heterologous
- Bacteria
- Central composite design
- Chemistry
- Heterologous expression
- Biochemistry
- Microbiology
- Biology
- Chromatography
- Recombinant DNA
- Bacillus subtilis
- Gene
- Genetics

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Sumber: Discover Unhas — RIMS Universitas Hasanuddin.
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