# In Vitro Selection of Salinity Tolerance Callus of Dwarf Napier Grass (Pennisetum purpureum cv. Mott)
> Khaerani P.

URL kanonis: https://discover.unhas.ac.id/publications/pub_scopus_85051385532
Jurnal / Konferensi: Iop Conference Series Earth and Environmental Science
Tahun terbit: 2018
DOI: https://doi.org/10.1088/1755-1315/175/1/012233
ISSN: 17551307
Citations: 1

## Authors
- Khaerani P.

## Abstract
Tissue culture for forage is a multiplication by improving the quality genetic of forage and adaptation to abiotic stress condition such as salinity stress. In this idea, different levels of salinity tolerance were generated. Multiple shoots as initial explants were isolated from aseptically shoot-tillers in the field, and cultured in vitro. Explants ware inducted into induction calli medium. Explants were transferred to Murashige Skoog (MS) medium, added with 5% coconut water and 2 mgL-1 2,4-D (dichlorophenoxyacetic acid). The planted explants were incubated for 21 days. Calli were induced in the proliferation medium with 50 μM CuSO4 and growth regulators i.e 0.1 mg L-1 2,4-D, and 2 mg L-1 BAP for 40 days. Regeneration medium with addition, and growth regulator 0.1 mg L-1 NAA and 2 mg L-1 BAP for 30 days. In the medium of proliferation and regeneration were added different salinity (NaCl) level. The treatments were 42.5 mM NaCl, 85 mM NaCl, 170 mM NaCl). The percentage of green-spot production, root formation, shoot, complete plantlet, morphologically of calli were recorded. The results indicated that morphologically and physiologically of calli added NaCl concentration (salinity) influenced growth on each treatment of dwarf napier grass (Pennisetum purpureum cv.Mott)

## Keywords
- Explant culture
- Salinity
- Shoot
- Biology
- Plantlet
- Pennisetum purpureum
- Murashige and Skoog medium
- Callus
- Horticulture
- Botany
- Forage
- Acclimatization
- In vitro
- Dry matter
- Ecology
- Biochemistry

---
Sumber: Discover Unhas — RIMS Universitas Hasanuddin.
Saat mengutip, gunakan DOI bila tersedia atau URL kanonis di atas.