Comparison of three inert markers in measuring apparent nutrient digestibility of juvenile abalone under different culture condition and temperature regimes

Abstract

A comparative research using three inert markers, chromic oxide, yttrium andytterbium to measure the apparent nutrient digestibility of experimental feed in juvenile Hybridabalone (<i>Haliotis rubra</i> X <i>H. laevigata</i>) and Greenlip abalone (<i>H.laevigata</i>) revealed thatapparent digestibility of crude protein (ADCP) measured using yttrium and ytterbium in hybridabalone were significantly different across the treatments. Protein digestibility measured inexperimental tanks was higher than those measured in indoor and outdoor commercial tanks,regardless of inert marker used. Chromic oxide led to overestimated AD<sub>CP</sub> compared to whenmeasured using yttrium and ytterbium. There were no significant interactions betweentemperature and inert markers when measuring AD<sub>CP</sub> and apparent digestibility of gross energy(AD<sub>GE</sub>). However, there was a significant difference of AD<sub>CP</sub> amongst inert markers whenmeasured in greenlip abalone cultured at two temperatures. While measurements of AD<sub>GE</sub>calculated using three inert markers shared the same value.

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