# Phylogenetic variation of chelonid alphaherpesvirus 5 (ChHV5) in populations of green turtles chelonia mydas along the Queensland Coast, Australia > Ariel E. URL kanonis: https://discover.unhas.ac.id/publications/pub_scopus_85042385176 Jurnal / Konferensi: Journal of Aquatic Animal Health Tahun terbit: 2017 DOI: https://doi.org/10.1080/08997659.2017.1330783 ISSN: 08997659 Kuartil SJR: Q2 Citations: 15 ## Authors - Ariel E. ## Abstract Sea turtle fibropapillomatosis (FP) is a disease marked by the proliferation of benign but debilitating cutaneous and occasional visceral tumors, likely to be caused by chelonid alphaherpesvirus 5 (ChHV5). This study presents a phylogeny of ChHV5 strains found on the east coast of Queensland, Australia, and a validation for previously unused primers. Two different primer sets (gB-1534 and gB-813) were designed to target a region including part of the UL27 glycoprotein B (gB) gene and part of UL28 of ChHV5. Sequences obtained from FP tumors found on juvenile green turtles Chelonia mydas (<65 cm curved carapace length) had substantial homology with published ChHV5 sequences, while a skin biopsy from a turtle without FP failed to react in the PCRs used in this study. The resulting sequences were used to generate a neighbor-joining tree from which three clusters of ChHV5 from Australian waters were identified: north Australian, north Queensland, and Queensland clusters. The clusters reflect the collection sites on the east coast of Queensland with a definitive north-south trend. Received October 22, 2016; accepted May 7, 2017. ## Keywords - Biology - Phylogenetic tree - Variation (astronomy) - Zoology - Fishery - Ecology - Gene - Genetics - Physics - Astrophysics --- Sumber: Discover Unhas — RIMS Universitas Hasanuddin. Saat mengutip, gunakan DOI bila tersedia atau URL kanonis di atas.