# Primer development to obtain complete coding sequence of HA and NA genes of influenza A/H3N2 virus
> Agustiningsih A.

URL kanonis: https://discover.unhas.ac.id/publications/pub_scopus_84984798002
Jurnal / Konferensi: BMC Research Notes
Tahun terbit: 2016
DOI: https://doi.org/10.1186/s13104-016-2235-8
ISSN: 17560500
Kuartil SJR: Q3
Citations: 5

## Authors
- Agustiningsih A.

## Abstract
BACKGROUND: Influenza is an acute respiratory illness and has become a serious public health problem worldwide. The need to study the HA and NA genes in influenza A virus is essential since these genes frequently undergo mutations. This study describes the development of primer sets for RT-PCR to obtain complete coding sequence of Hemagglutinin (HA) and Neuraminidase (NA) genes of influenza A/H3N2 virus from Indonesia. The primers were developed based on influenza A/H3N2 sequence worldwide from Global Initiative on Sharing All Influenza Data (GISAID) and further tested using Indonesian influenza A/H3N2 archived samples of influenza-like illness (ILI) surveillance from 2008 to 2009. RESULTS: An optimum RT-PCR condition was acquired for all HA and NA fragments designed to cover complete coding sequence of HA and NA genes. A total of 71 samples were successfully sequenced for complete coding sequence both of HA and NA genes out of 145 samples of influenza A/H3N2 tested. CONCLUSIONS: The developed primer sets were suitable for obtaining complete coding sequences of HA and NA genes of Indonesian samples from 2008 to 2009.

## Keywords
- Neuraminidase
- Gene
- Virology
- Primer (cosmetics)
- Influenza A virus
- Coding region
- RefSeq
- Virus
- Biology
- Sequence analysis
- GenBank
- Hemagglutinin (influenza)
- Genetics
- Complete sequence
- Genome
- Organic chemistry
- Chemistry

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Sumber: Discover Unhas — RIMS Universitas Hasanuddin.
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