# Terpenoids and a flavonoid glycoside from Acacia pennata leaves as hedgehog/GLI-mediated transcriptional inhibitors
> Rifai Y.

URL kanonis: https://discover.unhas.ac.id/publications/pub_scopus_77952970051
Jurnal / Konferensi: Journal of Natural Products
Tahun terbit: 2010
DOI: https://doi.org/10.1021/np1000818
ISSN: 01633864
Kuartil SJR: Q1
Citations: 69

## Authors
- Rifai Y.

## Abstract
Overexpression of glioma-associated oncogene 1 (GLI1), which has been characterized as a terminal effector and a target gene of the Hedgehog (Hh) signaling pathway, is associated with the development of cancer. A cellular screen was applied utilizing of a GLI-dependent luciferase reporter in human keratinocyte cells (HaCaT) and identified two terpenoids (1 and 2) and a flavonoid glycoside (5) from Acacia pennata as Hh/GLI inhibitors. Compounds 1, 2, and 5 exhibited selective cytotoxicity against human pancreatic (PANC1) and prostate (DU145) cancer cells with no toxic effect on normal cells. This result was consistent with a dose-dependent reduction of the protein levels of antiapoptotic BCL-2 and the tumor suppressor patched 1 protein (PTCH). Additionally, treatment of 1 downregulated mRNA expression of Ptch in PANC1, suggesting that the compound has an inhibitory effect on the transcription of Hh/GLI.

## Keywords
- GLI1
- Hedgehog
- Flavonoid
- Smoothened
- HaCaT
- DU145
- Hedgehog signaling pathway
- Terpenoid
- Biology
- Patched
- Chemistry
- Cancer research
- Biochemistry
- Cancer cell
- Pharmacology
- Cancer
- Signal transduction
- In vitro
- Antioxidant
- LNCaP
- Genetics

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Sumber: Discover Unhas — RIMS Universitas Hasanuddin.
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