# Early production of tumor necrosis factor-α by Gr-1+ cells and its role in the host defense to pneumococcal infection in lungs
> Hatta M.

URL kanonis: https://discover.unhas.ac.id/publications/pub_scopus_76349099573
Jurnal / Konferensi: FEMS Immunology and Medical Microbiology
Tahun terbit: 2010
DOI: https://doi.org/10.1111/j.1574-695X.2009.00616.x
ISSN: 09288244
Citations: 8

## Authors
- Hatta M.

## Abstract
In this study, we elucidated the role of tumor necrosis factor (TNF)-alpha in the host defense to pulmonary infection with Streptococcus pneumoniae and defined the cellular source of this cytokine at an early stage of infection. Administration of anti-TNF-alpha monoclonal antibody (mAb) resulted in the reduced accumulation of neutrophils in bronchoalveolar lavage fluids (BALFs) and severe exacerbation of this infection. In a flow cytometric analysis, the intracellular expression of TNF-alpha was detected in Gr-1(bright+) and Gr-1(dull+) cells during the time intervals postinfection, and F4/80(+) cells expressed intracellular TNF-alpha before Gr-1(dull+) cells appeared. The Gr-1(bright+) and Gr-1(dull+) cells sorted from BALF cells at 24 h were identified as neutrophils and macrophage-like cells, respectively, and the Gr-1(dull+) cells expressing CD11c, partially CD11b and a marginal level of F4/80 secreted TNF-alpha in in vitro cultures. Finally, deletion of Gr-1(+) cells by administration of the specific mAb significantly reduced the concentrations of this cytokine in BALF at 6 and 12 h postinfection, but not the expression of TNF-alpha in F4/80(+) cells. Thus, these results demonstrated that neutrophils, F4/80(+) macrophages and Gr-1(dull+) CD11c(+) macrophage-like cells played an important role in the production of TNF-alpha in lungs at an early stage of infection with S. pneumoniae.

## Keywords
- Tumor necrosis factor alpha
- Biology
- Cytokine
- Macrophage
- Monoclonal antibody
- Bronchoalveolar lavage
- CD11c
- Immunology
- Microbiology
- Molecular biology
- Antibody
- In vitro
- Lung
- Medicine
- Internal medicine
- Phenotype
- Gene
- Biochemistry

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Sumber: Discover Unhas — RIMS Universitas Hasanuddin.
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