# Quantitative determination of Plasmodium vivax gametocytes by real-time quantitative nucleic acid sequence-based amplification in clinical samples
> Beurskens M.

URL kanonis: https://discover.unhas.ac.id/publications/pub_scopus_68549099649
Jurnal / Konferensi: American Journal of Tropical Medicine and Hygiene
Tahun terbit: 2009
DOI: https://doi.org/10.4269/ajtmh.2009.81.366
ISSN: 00029637
Kuartil SJR: Q2
Citations: 23

## Authors
- Beurskens M.

## Abstract
Microscopic detection of Plasmodium vivax gametocytes, the sexual life stage of this malaria parasite, is insensitive because P. vivax parasitaemia is low. To detect and quantify gametocytes a more sensitive, quantitative real-time Pvs25-QT-NASBA based on Pvs25 mRNA was developed and tested in two clinical sample sets from three different continents. Pvs25-QT-NASBA is highly reproducible with low inter-assay variation and reaches sensitivity approximately 800 times higher than conventional microscopic gametocyte detection. Specificity was tested in 104 samples from P. vivax-, P. falciparum-, P. malariae-, and P. ovale-infected patients. All non-vivax samples were negative in the Pvs25-QT-NASBA; out of 74 PvS18-QT-NASBA positive samples 69% were positive in the Pvs25-QT-NASBA. In a second set of 136 P. vivax microscopically confirmed samples, gametocyte prevalence was 8%, whereas in contrast 66% were positive by Pvs25-QT-NASBA. The data suggest that the human P. vivax gametocyte reservoir is much larger when assessed by Pvs25-QT-NASBA than by microscopy.

## Keywords
- Gametocyte
- Plasmodium vivax
- Biology
- Virology
- Apicomplexa
- Parasite hosting
- Plasmodium malariae
- Plasmodium falciparum
- NASBA
- Malaria
- Nucleic acid sequence
- Immunology
- Genetics
- Gene
- World Wide Web
- Computer science

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Sumber: Discover Unhas — RIMS Universitas Hasanuddin.
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