# In Vitro mechanism-based inactivation of cytochrome P450 3A4 by a new constituent of Cinnamomum burmani
> Subehan

URL kanonis: https://discover.unhas.ac.id/publications/pub_scopus_54249151091
Jurnal / Konferensi: Planta Medica
Tahun terbit: 2008
DOI: https://doi.org/10.1055/s-2008-1081351
ISSN: 00320943
Kuartil SJR: Q2
Citations: 36

## Authors
- Subehan

## Abstract
Cytochrome P450 enzymes play an important role in drug metabolism. Various studies have reported the potential inhibition of these enzymes by natural compounds, leading to possible drug-herb interaction. One study reported that the MeOH extract of Cinnamomum burmani inhibited CYP3A4 in a mechanism-based mode. Further phytochemical investigation on this plant led to the isolation of 17 compounds including 2 new compounds: cinnamic aldehyde cyclic syringylglycerol 1,3-acetal (1) and 5'-hydroxy-5-hydroxymethyl-4'',5''-methylenedioxy-1,2,3,4-dibenzo-1,3,5-cycloheptatriene (2). The isolated constituents were tested for their preincubation time-dependent inhibition of CYP3A4 at 0 and 20 min. Only the new compound 2 showed an increase in the inhibitory activity to >50 % after 20-min preincubation. Further investigations of the inactivation by 2 displayed characteristics of a mechanism-based inactivator, with KI and kappainact values of 7.7 icroM and 0.04 min(-1), respectively.

## Keywords
- Cinnamomum
- CYP3A4
- Stereochemistry
- Methylenedioxy
- Chemistry
- Enzyme
- Cytochrome P450
- In vitro
- Hydroxymethyl
- Biochemistry
- Organic chemistry
- Cassia
- Medicine
- Alternative medicine
- Pathology
- Halogen
- Traditional Chinese medicine
- Alkyl

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Sumber: Discover Unhas — RIMS Universitas Hasanuddin.
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