# Derivation of hepato-pancreatic intermediate progenitor cells from a clonal mesenchymal stem cell line of rat bone marrow origin
> Masaka T.

URL kanonis: https://discover.unhas.ac.id/publications/pub_scopus_54049096702
Jurnal / Konferensi: International Journal of Molecular Medicine
Tahun terbit: 2008
DOI: https://doi.org/10.3892/ijmm_00000041
ISSN: 11073756
Kuartil SJR: Q1
Citations: 11

## Authors
- Masaka T.

## Abstract
We have recently established a clonal mesenchymal stem cell line (rBM25/S3) from adult rat bone marrow. The cells have practically unlimited proliferation capacity (over 300 PDL), maintaining multipotency for differentiation. In the present study, we examined the potential for rBM25/S3 cells to differentiate into insulin-secreting cells. When cultured in the presence of HGF and FGF-4 on Matrigel, rBM25/S3 cells expressed genes specific to pancreatic beta-cells as well as those specific to hepatocytes. They still maintained proliferation capacity with a doubling time of approximately 30 h. These hepato-pancreatic intermediate progenitor cells, but not the original undifferentiated rBM25/S3 cells, were induced by the overexpression of PDX-1 to produce significant amounts of insulin in a manner responding to glucose concentration in medium. The present culture system indicates a direction for further studies aimed at the realization of cell transplantation therapy for type I diabetes mellitus.

## Keywords
- Progenitor cell
- Mesenchymal stem cell
- Biology
- Stem cell
- Bone marrow
- Cell biology
- Stem cell transplantation for articular cartilage repair
- Matrigel
- Cancer research
- Clinical uses of mesenchymal stem cells
- Adult stem cell
- Cellular differentiation
- Endothelial stem cell
- Immunology
- In vitro
- Angiogenesis
- Gene
- Biochemistry

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Sumber: Discover Unhas — RIMS Universitas Hasanuddin.
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