# Induction of long-term lipopolysaccharide tolerance by an agonistic monoclonal antibody to the toll-like receptor 4/MD-2 complex
> Ohta S.

URL kanonis: https://discover.unhas.ac.id/publications/pub_scopus_33750327329
Jurnal / Konferensi: Clinical and Vaccine Immunology
Tahun terbit: 2006
DOI: https://doi.org/10.1128/CVI.00173-06
ISSN: 15566811
Citations: 27

## Authors
- Ohta S.

## Abstract
We have established an agonistic monoclonal antibody, UT12, that induces stimulatory signals comparable to those induced by lipopolysaccharide (LPS) through Toll-like receptor 4 and MD-2. UT12 activated nuclear factor kappaB and induced the production of proinflammatory cytokines such as tumor necrosis factor alpha (TNF-alpha) and interleukin-6 (IL-6) in peritoneal exudative cells. In addition, mice injected with UT12 rapidly fell into endotoxin shock concomitant with the augmentation of serum TNF-alpha and IL-6 levels, followed by death within 12 h. On the other hand, when the mice were pretreated with a sublethal dose of UT12, the mice survived the subsequent lethal LPS challenges, with significant suppression of serum TNF-alpha and IL-6, indicating that UT12 induced tolerance against LPS. This effect of UT12 was maintained for at least 9 days. In contrast, the tolerance induced by LPS continued for less than 3 days. These results illuminate a novel potential therapeutic strategy for endotoxin shock by the use of monoclonal antibodies against the Toll-like receptor 4/MD-2 complex.

## Keywords
- Lipopolysaccharide
- Monoclonal antibody
- Proinflammatory cytokine
- Tumor necrosis factor alpha
- Toll-like receptor
- Receptor
- Immunology
- Antibody
- Biology
- Medicine
- Inflammation
- Internal medicine
- Innate immune system

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Sumber: Discover Unhas — RIMS Universitas Hasanuddin.
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