# Different roles of N-terminal and C-terminal halves of HIRA in transcription regulation of cell cycle-related genes that contribute to control of vertebrate cell growth
> Ahmad A.

URL kanonis: https://discover.unhas.ac.id/publications/pub_scopus_25444478878
Jurnal / Konferensi: Journal of Biological Chemistry
Tahun terbit: 2005
DOI: https://doi.org/10.1074/jbc.M501426200
ISSN: 00219258
Kuartil SJR: Q1
Citations: 16

## Authors
- Ahmad A.

## Abstract
We reported previously that chicken HIRA, a homolog of Saccharomyces cerevisiae transcriptional co-repressors Hir1p and Hir2p, possesses seven WD dipeptide motifs and an LXXLL motif in its N-terminal and C-terminal halves, respectively, required for transcription regulations. Here, by using the gene targeting technique, we generated the homozygous HIRA-deficient DT40 mutant DeltaHIRA. The HIRA deficiency caused slightly delayed cell growth and affected the opposite transcriptions of cell cycle-related genes, i.e. repressions for P18, CDC25B, and BCL-2, activations for P19 and cyclin A, and histones H2A, H2B, H3, and H4. These altered expressions were completely revived by the artificial stable expression of hemagglutinin-tagged HIRA in DeltaHIRA. The ability to rescue the delayed growth rate was preferentially aided by the N-terminal half instead of the C-terminal half. We cloned the chicken P18 genomic DNA, and we established that its promoter was located surrounding the sequence GCGGGCGC at positions -1157 to -1150. Chromatin immunoprecipitation assay revealed that the N-terminal half interacted directly or indirectly with the putative promoter region of the p18 gene, resulting in up-regulation of the gene. These results indicated that the N-terminal half of HIRA should contribute positively to the growth rate via up-regulation of a set of cell cycle-related genes, whereas the C-terminal half down-regulated another set of them without exhibiting any effect on the cell growth.

## Keywords
- Gene
- Biology
- Repressor
- Chromatin immunoprecipitation
- Cell cycle
- Transcriptional regulation
- Chromatin
- Transcription (linguistics)
- Genetics
- Transcription factor
- Molecular biology
- Gene expression
- Promoter
- Philosophy
- Linguistics

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Sumber: Discover Unhas — RIMS Universitas Hasanuddin.
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