# A Bright Ratiometric Dipyrene Probe for Functional Imaging of Condensate Microenvironments in Living Cells
> Matsumoto K.

URL kanonis: https://discover.unhas.ac.id/publications/pub_scopus_105045152242
Jurnal / Konferensi: Advanced Science
Tahun terbit: 2026
DOI: https://doi.org/10.1002/advs.76419
ISSN: 21983844
Kuartil SJR: Q1
Citations: 0

## Authors
- Matsumoto K.

## Abstract
Biomolecular condensates exhibit spatially heterogeneous microenvironments shaped by intertwined physicochemical factors, yet practical small-molecule tools for mapping these states in living cells remain limited. Here, we report PyLUMI, a bright dipyrene ratiometric probe, optimized by linker engineering to improve fluorescence output under aqueous conditions while preserving excimer-to-monomer (E/M) readout capability. Solution-phase characterization identifies a scaffold with enhanced fluorescence efficiency and an E/M response to coupled polarity-viscosity microenvironmental changes. In recombinant protein condensates, PyLUMI provides substantially increased signal and enables pixel-resolved visualization of intra-condensate heterogeneity. In living cells, the probe enables low-exposure ratiometric imaging of centrosome-associated condensate compartments and reveals microenvironmental signatures linked to cell-cycle progression and pathology-associated centrosome amplification beyond morphological size differences. These results establish PyLUMI as a functional chemical reporter for spatially resolved profiling of integrated physicochemical fingerprints in condensate-associated compartments.

## Keywords
- Linker
- Fluorescence
- Chemistry
- Biophysics
- Fluorescence-lifetime imaging microscopy
- Characterization (materials science)
- Nanotechnology
- Centrosome
- High resolution
- Optical imaging
- Scaffold
- Preclinical imaging
- Functional imaging
- Förster resonance energy transfer
- Living cell
- Biological imaging
- Click chemistry
- Molecular imaging
- Aqueous solution
- Direct imaging
- Live cell imaging
- Tissue engineering

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Sumber: Discover Unhas — RIMS Universitas Hasanuddin.
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