IDENTIFICATION OF THERMOPHILIC BACTERIA BACILLUS BADIUS W.IIISRNA_2.1 IS A POTENTIALLY NOVEL STRAIN FROM MATAUMPANA HOT SPRINGS, BUTON, INDONESIA, AS PRODUCERS OF URICASE ENZYME

Abstract

Thermophilic bacteria are unique microorganisms because they can survive and thrive in high temperatures and extreme environments such as hot springs. Microorganisms that survive in environment like this contain active compounds, one of which is the enzyme uricase. Uricase is an enzyme that plays an important role in the nitrogen metabolism pathway to catalyze oxidation of uric acid into allantoin, CO2 and H2O2 which are water soluble easily. This study aims to identify thermophilic bacterial species producers of uricase enzyme isolated from Mataumpana hot spring water samples and determine the effect of incubation time on bacterial growth and uricase enzyme production. Bacterial identification includes morphological and biochemical tests as well as species-level molecular tests using 16S rRNA, then determining the effect of incubation time on bacterial growth and uricase enzyme production. The results of the analysis showed that the uricase-producing thermophilic bacterial isolate W.IIISRNa_2.1 strain was identified as coming from the gram-positive Bacillus genus and had a very close relationship with the Bacillus badius species with a similarity of 98.97%, so the isolate was named Bacillus badius W.IIISRNa_2.1. This bacterium has an optimal optical density (OD) at 42 hours of incubation of 0.962, which is different from the incubation time for production of uricase, was 12 hours with an enzyme activity of 0.4928 U/ml and a protein concentration of 4.7715 mg/ml.

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