# T2T Genome Assembly and Multi-Omics Data Reveal Terrestrial Adaptation and Mucus Biosynthesis in Tropical Leatherleaf Slug (Laevicaulis alte)
> Wang G.

URL kanonis: https://discover.unhas.ac.id/publications/pub_scopus_105041634858
Jurnal / Konferensi: Advanced Science
Tahun terbit: 2026
DOI: https://doi.org/10.1002/advs.76129
ISSN: 21983844
Kuartil SJR: Q1
Citations: 0

## Authors
- Wang G.

## Abstract
Laevichaulis alte is a slug in the order Systellommatophora that evolved from aquatic ancestors and now faces strong challenges from desiccation, respiration on land, and novel pathogens. Its mucus is essential for water retention, locomotion, and defense. To link terrestrial adaptation with mucus biosynthesis, we generated a gap-free genome assembly of L. alte using PacBio HiFi reads, Oxford Nanopore ultra-long reads, and Hi-C data. The genome shows low heterozygosity and holocentromeric chromosomes. Functional metabolomics revealed marked metabolic shifts between L. alte and the closely related aquatic species Peronia verruculata. In L. alte, differential metabolites were enriched in lipid metabolism, immune regulation, and stress response pathways, consistent with life in a dry and microbe-rich terrestrial environment. Comparative genomics and transcriptomics identified candidate genes linked to mucus secretion and physiological adaptation, including VEGF, ASGR2, and COL6A6. Further analyses highlighted the vascular endothelial growth factor (VEGF) gene family as a key regulator connecting angiogenesis, tissue remodeling, and mucus production pathways in L. alte. Together, this gap-free genome and multi-omics dataset establish a molecular framework that links genomic innovation, mucus biology, and terrestrial adaptation in Systellommatophora, and they offer a basis for understanding ecological niche specialization in land molluscs.

## Keywords
- Biology
- Genome
- Adaptation (eye)
- Transcriptome
- Mucus
- Genomics
- Gene
- Comparative genomics
- Sequence assembly
- Genetics
- Gene family
- Evolutionary biology
- Metabolomics
- Slug
- Functional genomics
- Phylogenetic tree
- Computational biology
- Niche
- Regulator
- Candidate gene
- Proteomics
- Regulation of gene expression
- Phylogenetics
- Metabolic pathway

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Sumber: Discover Unhas — RIMS Universitas Hasanuddin.
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