# Antihistamine Potential of Red Alga Acanthophora spicifera Through Combination In Vivo, In Vitro, and In Silico Study
> Wahyuni I.

URL kanonis: https://discover.unhas.ac.id/publications/pub_scopus_105034991634
Jurnal / Konferensi: Indonesian Journal of Chemistry
Tahun terbit: 2026
DOI: https://doi.org/10.22146/ijc.109199
ISSN: 14119420
Kuartil SJR: Q3
Citations: 0

## Authors
- Wahyuni I.

## Abstract
Red alga Acanthophora spicifera is a marine species with potential as a source of natural antihistamines. This study evaluated the bioactivity of its secondary metabolites through in vivo, in vitro, and in silico approaches. Extracts were obtained by successive maceration using n-hexane, ethyl acetate, and methanol. In vivo testing on BALB/c mice showed that extract administration did not cause significant weight loss. However, its effect on allergic symptoms was significant (p < 0.05), with the methanol extract identified as the most effective by the Mann–Whitney test. In vitro assays revealed a significant reduction in histamine levels (p < 0.05) during sensitization, treatment, and post-challenge phases, particularly in the methanol extract group. In silico docking demonstrated that two compounds from the methanol extract exhibited strong binding affinity to the H1 receptor (PDB ID: 3RZE), with binding energies of −6.27 and −5.06 kcal/mol. These findings suggest that A. spicifera, particularly its methanol extract, is a promising source of natural antihistamines with potential applications in the development of safer allergy treatments.

## Keywords
- Chemistry
- Antihistamine
- In silico
- Methanol
- Maceration (sewage)
- In vivo
- Histamine
- In vitro
- Pharmacology
- Biochemistry
- Biological activity
- Docking (animal)
- Traditional medicine
- Chromatography
- Histamine receptor
- Carbenoxolone
- Terfenadine
- In vitro toxicology
- Receptor

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Sumber: Discover Unhas — RIMS Universitas Hasanuddin.
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