# Synergistic photodynamic antimicrobial therapy with silver nanoparticle activated by UV irradiation and 405 nm laser diode against Escherichia coli and Staphylococcus aureus
> Astuti S.D.

URL kanonis: https://discover.unhas.ac.id/publications/pub_scopus_105033730827
Jurnal / Konferensi: Brazilian Journal of Biology
Tahun terbit: 2026
DOI: https://doi.org/10.1590/1519-6984.299835
ISSN: 15196984
Kuartil SJR: Q2
Citations: 0

## Authors
- Astuti S.D.

## Abstract
This study investigates the antibacterial properties of Moringa oleifera green-synthesized silver nanoparticles (AgNPs-MO) against Escherichia coli (E. coli) and Staphylococcus aureus (S. aureus) when combined with UV radiation and a blue laser set at 405 nm. Exposure to both light treatments increased the antibacterial activity of AgNPs-MO at higher concentrations. The results indicate that photosensitizers generated from Moringa oleifera help photodynamic antimicrobial treatment work better and provide a more environmentally friendly way to manage infections. A control group (T0) was not subjected to laser irradiation, while the other three bacterial groups (A1/A2 and A3/A4) were treated to laser irradiation at different concentrations and times of silver nanoparticles. Groups A2 and A4 bacteria were also given a photosensitizer made from Moringa oleifera, and then they were exposed to varying lengths of laser light. Following incubation, a Quebec colony counter was used to measure the number of bacterial colonies. Bacterial viability was significantly reduced, according to statistical analysis utilizing a post-hoc Tukey test and a two-way ANOVA factorial test. After being exposed to mmol L-1, mmol L-1.5, and mmol L-2 AgNPs-MO for 180 seconds, E. coli showed viability decreases of 70.90%, 76.44%, and 79.87%, respectively. The identical circumstances also resulted in viability decreases of 97.48%, 95.94%, and 94.72% for S. aureus. Our strategy proved to be effective, inactivating E. coli by 79.87% ± 1.92% and S. aureus by 97.48% ± 0.78% at an energy density of 3.44 J/cm2. In order to battle pathogenic germs, this research proposes prospective uses for a blue laser, UV light, silver nanoparticles, and extract from Moringa oleifera.

## Keywords
- Staphylococcus aureus
- Escherichia coli
- Moringa
- Antimicrobial
- Photosensitizer
- Silver nanoparticle
- Photodynamic therapy
- Microbiology
- Antibacterial activity
- Biology
- Bacteria
- Food science
- Nuclear chemistry
- Irradiation
- Pathogenic bacteria
- Photosensitivity
- Laser
- Factorial experiment
- Pathogen
- MTT assay
- In vivo

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Sumber: Discover Unhas — RIMS Universitas Hasanuddin.
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