The Inhibition of A549 cells from Siam Weeds (Chromolaena odorata L.)

Abstract

Introduction: Lung cancer is the health problem in developed countries. The main causes of lung cancer in general include carcinogens such as tobacco smoke, ionizing radiation, and viral infections. This exposure causes cumulative changes to the DNA in the tissue lining the bronchial epithelium. Methods: Chromolaena odorata was extracted with methanol, then partitioned with 5 dissolutions; n-Hexane, Ethyl Acetate, Acetonitric, n-Buthanol, Ethanol. The five extracts obtained were tested with A549 cells using the bioassay method. After incubation, the result obtained from microplate reader. Result: The percentage inhibition of the five extracts was showed that the Ethanol extract was the most active, with the highest value among other solvents. The A549 cell line was isolated in 1973 from lung adenocarcinoma in a study to create a continuous cell line from 200 different tumors. Although developed as a tool for cancer research, the cell line was later characterized as representative of the Alveolar Type II pneumocytes from the human lung. The cell line has been a mainstay of respiratory research for nearly forty years. Conclusion: The Ethanol extract of Chromolaena odorata has a potential to inhibit the growth of the A549 cell line

Access to Document

Other files and links

• Link to publication in Scopus

Fingerprint