Selection of dominant marker primers and annealing optimum temperatures for Syzygium polycephalum from UNHAS educational forest

Abstract

Abstract DNA amplification using polymorphic primers can be used for analyzing the genetic diversity of Syzygium polycephalum . Primers from dominant markers such as RAPD and ISSR can be used for this analysis. Primer information is needed that can amplify Syzygium polycephalum DNA and produce polymorphic bands, so that this study aims to select 10 RAPD primers (OPA 11, OPG 06, PLR 13, PLW 04, PLC 14, PLB 10, M 147, M 29, PLD 08, M 33) and 10 ISSR primers (UBC 810, UBC 868, UBC 827, UBC 824, UBC 823, UBC 822, UBC 830, UBC 820, UBC 814, UBC 813). Of the 10 RAPD primers selected, eight primers were able to amplify Syzygium polycephalum DNA and produce polymorphic bands. One primer produced monomorphic bands (PLC 14), and one primer (PLW 04) was only able to amplify less than 50% of the samples tested. As for ISSR markers, all primers produced polymorphic bands. RAPD primers have a lower optimum annealing temperature with a smaller range than ISSR primers, where the optimum annealing temperature of RAPD primers ranges from 31.4 to 34.6°C, while ISSR is between 43.4 and 53.4°C. Primers from RAPD and ISSR markers that produce polymorphic bands, as indicated by the research results, can be used to analyze the genetic diversity of Syzygium polycephalum.

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