# Computer-Assisted Semen Analysis in Indonesian Buffalo: Correlations with Plasma Membrane Integrity, DNA Fragmentation, and Acrosome Integrity
> Diansyah A.M.

URL kanonis: https://discover.unhas.ac.id/publications/pub_scopus_105023185891
Jurnal / Konferensi: Bio Web of Conferences
Tahun terbit: 2025
DOI: https://doi.org/10.1051/bioconf/202518901024
ISSN: 22731709
Citations: 0

## Authors
- Diansyah A.M.

## Abstract
This research investigated the association between structural quality and motility traits of cryopreserved semen in Silangit and Toraya buffalo using a computer-assisted semen analysis (CASA) system. Post-thaw examination revealed no statistical difference in plasma membrane integrity between the two breeds (Silangit: 62.5 ± 3.2%; Toraya: 63.1 ± 2.8%). In contrast, the level of DNA fragmentation was greater in Silangit bulls (18.4 ± 2.6%) compared with Toraya (12.7 ± 1.9%). Acrosome condition was generally well maintained in both groups (>95%). The CASA assessment further revealed that Toraya sperm exhibited superior values for average path velocity and lateral head displacement, while Silangit samples displayed higher straightness, linearity, and beat cross frequency. Overall, the study demonstrates that differences in membrane stability, DNA integrity, and acrosome status contribute to breed-specific motility profiles, emphasizing the relevance of CASA for designing semen preservation strategies in buffalo breeding.

## Keywords
- Acrosome
- Semen
- Sperm
- DNA fragmentation
- Andrology
- Semen quality
- Membrane integrity
- Semen analysis
- Biology
- Motility
- DNA
- Sperm motility
- Acrosome reaction
- Cryopreservation
- Statistical analysis
- Chemistry
- Membrane
- Sperm quality
- Fragmentation (computing)
- Human fertilization
- Significant difference
- Molecular biology

---
Sumber: Discover Unhas — RIMS Universitas Hasanuddin.
Saat mengutip, gunakan DOI bila tersedia atau URL kanonis di atas.